Recombinant Human METTL3/METTL14 complex, N-FLAG-tagged

• Cat.No.: METTL14-34H
• Product Overview: Recombinant METTL3 / METTL14 complex that includes full length human METTL3 (accession number NP_062826.2) without tag and full length METTL14 protein (accession number NP_066012.1) with an N-terminal FLAG-Tag was expressed in Sf9 cells.

Specification

• Description: N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs).
  In mammalian cells, METTL3 (methyltransferase-like 3, also known as IME4, M6A, MT-A70) and METTL14 (methyltransferase-like 14) are two m6A methyltransferases reported to date, which both contain methyltransferase domains. These two proteins form a stable heterodimer core complex of METTL3–METTL14 that functions in cellular m6A deposition on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition.
• Source: Baculovirus
• Species: Human
• Tag: N-FLAG
• Molecular Mass: METTL3 and METTL14 are 64.5 kDa and 53.3 kDa, respectively.
• Purity: >95%
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Gene Information

• Gene Name: METTL14 methyltransferase 14, N6-adenosine-methyltransferase subunit [ Homo sapiens (human) ]
• Official Symbol: METTL15
• Synonyms: METTL14; methyltransferase 14, N6-adenosine-methyltransferase subunit; hMETTL14
• Gene ID: 57721
• mRNA Refseq: NM_020961
• Protein Refseq: NP_066012
• MIM: 616504
• UniProt ID: Q9HCE5