Active Recombinant Human MOK(1-343) Protein, N-GST,C-His-tagged

• Cat.No.: MOK-156H
• Product Overview: N-terminal GSTtagged and C-terminal 6His tagged, recombinant, human MOK amino acids 1- 343 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography followed by glutathione agarose.

Specification

• Description: MOK is able to phosphorylate several exogenous substrates and to undergo autophosphorylation. Negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner.
• Source: Sf21
• Species: Human
• Tag: GST,His
• Protein length: 1-343 aa
• Form: 0.17mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 410U/mg, where one unit of MOK activity is defined as 1nmol phosphate incorporated into 300μM RSRSRSRSRSRSRSR per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 70 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFM KNYKAIGKIG 241 EGTFSEVMKM QSLRDGNYYA CKQMKQRFES IEQVNNLREI QALRRLNPHP NILMLHEVVF 301 DRKSGSLALI CELMDMNIYE LIRGRRYPLS EKKIMHYMYQ LCKSLDHIHR NGIFHRDVKP 361 ENILIKQDVL KLGDFGSCRS VYSKQPYTEY ISTRWYRAPE CLLTDGFYTY KMDLWSAGCV 421 FYEIASLQPL FPGVNELDQI SKIHDVIGTP AQKILTKFKQ SRAMNFDFPF KKGSGIPLLT 481 TNLSPQCLSL LHAMVAYDPD ERIAAHQALQ HPYFQEQRKT EKRALGSHRK AGFPEHPVAP 541 EPLSNSCQIS KEGRKQKQSL KQEEDRPKRR GPENLYFQGV EACQLGTDDY DIPTTHHHHH 601 H
• Purity: Purity 88% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MOK MOK protein kinase [ Homo sapiens (human) ]
• Official Symbol: MOK
• Synonyms: MOK; MOK protein kinase; RAGE; RAGE1; STK30; RAGE-1
• Gene ID: 5891
• mRNA Refseq: NM_001272011.2
• Protein Refseq: NP_001258940.1
• MIM: 605762
• UniProt ID: Q9UQ07