Active Recombinant Human NR2C2(1-303) and TAB1(437-end) fused Protein, N-His-tagged

• Cat.No.: NR2C2-TAB1-236H
• Product Overview: N-terminal 6His-tagged, recombinant, human TAK1 amino acids 1–303, fused to human TAB1 amino acids 437–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: TAK1-TAB1 is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-303 aa and 437-end
• Form: 2.231mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 72U/mg, where one unit of TAK1-TAB fusion activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 42.8 kDa
• AA Sequence: 1 MGHHHHHHMS TASAASSSSS SSAGEMIEAP SQVLNFEEID YKEIEVEEVV GRGAFGVVCK 61 AKWRAKDVAI KQIESESERK AFIVELRQLS RVNHPNIVKL YGACLNPVCL VMEYAEGGSL 121 YNVLHGAEPL PYYTAAHAMS WCLQCSQGVA YLHSMQPKAL IHRDLKPPNL LLVAGGTVLK 181 ICDFGTACDI QTHMTNNKGS AAWMAPEVFE GSNYSEKCDV FSWGIILWEV ITRRKPFDEI 241 GGPAFRIMWA VHNGTRPPLI KNLPKPIESL MTRCWSKDPS QRPSMEEIVK IMTHLMRYFP 301 GADEPLQYPC QEFGGGGGQS PTLTLQSTNT HTQSSSSSSD GGLFRSRPAH SLPPGEDGRV 361 EPYVDFAEFY RLWSVDHGEQ SVVTAP
• Purity: Purity 88% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: NR2C2 nuclear receptor subfamily 2 group C member 2 [ Homo sapiens (human) ] and TAB1 TGF-beta activated kinase 1 (MAP3K7) binding protein 1 [ Homo sapiens (human) ]
• Official Symbol: NR2C2 and TAB1
• Synonyms: NR2C2 and TAB1; nuclear receptor subfamily 2 group C member 2 and TGF-beta activated kinase 1 (MAP3K7) binding protein 1; TR4; TAK1 and 3'-Tab1; MAP3K7IP1
• Gene ID: 7182 and 10454
• mRNA Refseq: NM_003298.5 and NM_006116.3
• Protein Refseq: NP_003289.2 and NP_006107.1
• MIM: 601426 and 602615
• UniProt ID: P49116 and Q15750