Active Recombinant Human NUAK1(2-end) Protein, N-His-tagged

• Cat.No.: NUAK1-24H
• Product Overview: N-terminal 6Histagged, recombinant, human ARK5, amino acids 2–end expressed by baculovirus in Sf21 cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: ARK5 (AMPK related kinase 5) is a serine/threonine kinase and a member of the AMPK family of protein kinases. In response to stimulation by Akt, ARK5 prevents cell death induced by glucose starvation and death receptor activation in tumor cells as a result of inhibition of caspase 8 activation via preservation of c-FLIP. ARK5 can also negatively regulate procaspase-6 via direct phosphorylation at Ser257, leading to resistance to the FasL/Fas signaling system.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 0.489mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 273U/mg, where one unit of ARK5, active activity is defined as 1nmol phosphate incorporated into 300μM (KKKVSRSGLYRSPSMPENLNRPR) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 78 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF EGAAAPVAGD RPDLGLGAPG SPREAVAGAT 61 AALEPRKPHG VKRHHHKHNL KHRYELQETL GKGTYGKVKR ATERFSGRVV AIKSIRKDKI 121 KDEQDMVHIR REIEIMSSLN HPHIISIYEV FENKDKIVII MEYASKGELY DYISERRRLS 181 ERETRHFFRQ IVSAVHYCHK NGVVHRDLKL ENILLDDNCN IKIADFGLSN LYQKDKFLQT 241 FCGSPLYASP EIVNGRPYRG PEVDSWALGV LLYTLVYGTM PFDGFDHKNL IRQISSGEYR 301 EPTQPSDARG LIRWMLMVNP DRRATIEDIA NHWWVNWGYK SSVCDCDALH DSESPLLARI 361 IDWHHRSTGL QADTEAKMKG LAKPTTSEVM LERQRSLKKS KKENDFAQSG QDAVPESPSK 421 LSSKRPKGIL KKRSNSEHRS HSTGFIEGVV GPALPSTFKM EQDLCRTGVL LPSSPEAEVP 481 GKLSPKQSAT MPKKGILKKT QQRESGYYSS PERSESSELL DSNDVMGSSI PSPSPPDPAR 541 VTSHSLSCRR KGILKHSSKY SAGTMDPALV SPEMPTLESL SEPGVPAEGL SRSYSRPSSV 601 ISDDSVLSSD SFDLLDLQEN RPARQRIRSC VSAENFLQIQ DFEGLQNRPR PQYLKRYRNR 661 LADSSFSLLT DMDDVTQVYK QALEICSKLN
• Purity: Purity 87% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: NUAK1 NUAK family kinase 1 [ Homo sapiens (human) ]
• Official Symbol: NUAK1
• Synonyms: NUAK1; NUAK family kinase 1; ARK5
• Gene ID: 9891
• mRNA Refseq: NM_014840.3
• Protein Refseq: NP_055655.1
• MIM: 608130
• UniProt ID: O60285