| Description : |
PDGFRα (Platelet-Derived Growth Factor Receptor Alpha) is a transmembrane receptor protein that belongs to the family of receptor tyrosine kinases. It is involved in a variety of cellular processes, including cell growth, differentiation, migration, and survival. PDGFRα is expressed in a variety of tissues and cell types, including fibroblasts, smooth muscle cells, and certain types of cancer cells. Aberrant activation of PDGFRα has been implicated in several diseases, including cancer, fibrotic disorders, and vascular diseases. PDGFRα mutations, amplifications, and overexpression have been found in various types of cancer, making it a potential target for cancer therapy. Additionally, drugs that target PDGFRα have been approved for the treatment of certain types of cancer, such as gastrointestinal stromal tumors (GIST) and chronic myelomonocytic leukemia (CMML). |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
550-end |
| Form : |
2.79mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
705U/mg, where one unit of PDGFR alpha (V561D), active activity is defined as 1nmol phosphate incorporated into 250μM (GGMEDIYFEFMGGKKK) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
63.5 kDa |
| AA Sequence : |
1 MAHHHHHHEN LYFQGKQKPR YEIRWRDIES ISPDGHEYIY VDPMQLPYDS RWEFPRDGLV 61 LGRVLGSGAF GKVVEGTAYG LSRSQPVMKV AVKMLKPTAR SSEKQALMSE LKIMTHLGPH 121 LNIVNLLGAC TKSGPIYIIT EYCFYGDLVN YLHKNRDSFL SHHPEKPKKE LDIFGLNPAD 181 ESTRSYVILS FENNGDYMDM KQADTTQYVP MLERKEVSKY SDIQRSLYDR PASYKKKSML 241 DSEVKNLLSD DNSEGLTLLD LLSFTYQVAR GMEFLASKNC VHRDLAARNV LLAQGKIVKI 301 CDFGLARDIM HDSNYVSKGS TFLPVKWMAP ESIFDNLYTT LSDVWSYGIL LWEIFSLGGT 361 PYPGMMVDST FYNKIKSGYR MAKPDHATSE VYEIMVKCWN SEPEKRPSFY HLSEIVENLL 421 PGQYKKSYEK IHLDFLKSDH PAVARMRVDS DNAYIGVTYK NEEDKLKDWE GGLDEQRLSA 481 DSGYIIPLPD IDPVPEEEDL GKRNRHSSQT SEESAIETGS SSSTFIKRED ETIEDIDMMD 541 DIGIDSSDLV EDSFL |
| Purity : |
Purity 81% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
| References : |
Hirota S et al., Gastroenterology (2003); 125:660-667 and Lasota, J. et al., Laboratory Investigation (2004): 84: 874-883. |
