Active Recombinant Human PDK1(52-end) Protein, N-His-tagged

• Cat.No.: PDK1-188H
• Product Overview: N-terminal, 6Histagged, recombinant human PDK1, amino acids 52–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: Phosphoinositide-Dependent Kinase-1 (PDK1) plays a central role in mediating cell signaling between PI3K and various intracellular serine/threonine kinases by phosphorylating many other kinases including PKC, p70S6 kinase, ribosomal S6 kinase, p21-activated kinase, PKC-related kinase, PKB and serum glucocorticoid kinase. PDK1 interacts with a wide range of substrates, and has been found to regulate apoptosis, cell proliferation and survival through the PI3K signaling pathway. A PDK1 inhibitor may be of therapeutic use in the treatment of human cancers and diabetes.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 52-end
• Form: 2.69mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 5% glycerol, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 188U/mg, where one unit of PDK1 activity is defined as 1nmol phosphate incorporated into 100μM PDKtide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 59 kDa
• AA Sequence: 1 MHHHHHHDGT AAEPRPGAGS LQHAQPPPQP RKKRPEDFKF GKILGEGSFS TVVLARELAT 61 SREYAIKILE KRHIIKENKV PYVTRERDVM SRLDHPFFVK LYFTFQDDEK LYFGLSYAKN 121 GELLKYIRKI GSFDETCTRF YTAEIVSALE YLHGKGIIHR DLKPENILLN EDMHIQITDF 181 GTAKVLSPES KQARANSFVG TAQYVSPELL TEKSACKSSD LWALGCIIYQ LVAGLPPFRA 241 GNEYLIFQKI IKLEYDFPEK FFPKARDLVE KLLVLDATKR LGCEEMEGYG PLKAHPFFES 301 VTWENLHQQT PPKLTAYLPA MSEDDEDCYG NYDNLLSQFG CMQVSSSSSS HSLSASDTGL 361 PQRSGSNIEQ YIHDLDSNSF ELDLQFSEDE KRLLLEKQAG GNPWHQFVEN NLILKMGPVD 421 KRKGLFARRR QLLLTEGPHL YYVDPVNKVL KGEIPWSQEL RPEAKNFKTF FVHTPNRTYY 481 LMDPSGNAHK WCRKIQEVWR QRYQSHPDAA VQ
• Purity: Purity 100% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PDK1 pyruvate dehydrogenase kinase 1 [ Homo sapiens (human) ]
• Official Symbol: PDK1
• Synonyms: PDK1 ; pyruvate dehydrogenase kinase 1; PDPK1; MGC20087; PkB-like; PRO0461; MGC35290; hPDK1
• Gene ID: 5163
• mRNA Refseq: NM_002610.5
• Protein Refseq: NP_002601.1
• MIM: 602524
• UniProt ID: Q15118