Active Recombinant Human PKN2(501-end) Protein, N-His-tagged

• Cat.No.: PKN2-203H
• Product Overview: Recombinant human PRK2, residues 501–end, containing Nterminal 6His tag. Expressed by baculovirus in Sf21 insect cells and purified by Ni2+/NTA-agarose.

Specification

• Description: PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-foes serum response factor (SRF). Involved in the negative regulation of ciliogenesis.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 501-end
• Form: 0.63mg/ml of enzyme in 50mM Tris/HCl pH7.5, 270mM sucrose, 150mM NaCl, 1mM benzamidine, 0.1mM EGTA, 0.1 % 2-mercaptoethanol, 0.03% Brij-35.
• Bio-activity: 202U/mg, where one unit equals the incorporation of 1nmol of phosphate per min into 30μM synthetic substrate peptide (AKRRRLSSLRA) at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 59.9 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSAT MDYKDDDDKG KTFLRAPQMN INIATWGRLV 61 RRAIPTVNHS GTFSPQAPVP TTVPVVDVRI PQLAPPASDS TVTKLDFDLE PEPPPAPPRA 121 SSLGEIDESS ELRVLDIPGQ DSETVFDIQN DRNSILPKSQ SEYKPDTPQS GLEYSGIQEL 181 EDRRSQQRFQ FNLQDFRCCA VLGRGHFGKV LLAEYKNTNE MFAIKALKKG DIVARDEVDS 241 LMCEKRIFET VNSVRHPFLV NLFACFQTKE HVCFVMEYAA GGDLMMHIHT DVFSEPRAVF 301 YAACVVLGLQ YLHEHKIVYR DLKLDNLLLD TEGFVKIADF GLCKEGMGYG DRTSTFCGTP 361 EFLAPEVLTE TSYTRAVDWW GLGVLIYEML VGESPFPGDD EEEVFDSIVN DEVRYPRFLS 421 TEAISIMRRL LRRNPERRLG ASEKDAEDVK KHPFFRLIDW SALMDKKVKP PFIPTIRGRE 481 DVSNFDDEFT SEAPILTPPR EPRILSEEEQ EMFRDFDYIA DWC
• Purity: Purity 71.9% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PKN2 protein kinase N2 [ Homo sapiens (human) ]
• Official Symbol: PKN2
• Synonyms: PKN2; protein kinase N2; PAK2; PRK2; STK7; Pak-2; PRKCL2; PRO2042
• Gene ID: 5586
• mRNA Refseq: NM_006256.4
• Protein Refseq: NP_006247.1
• MIM: 602549
• UniProt ID: Q16513