Active Recombinant Human PRKCB(2-end) Protein, N-His-tagged

• Cat.No.: PRKCB-195H
• Product Overview: N-Terminal 6Histagged, recombinant, human PKC beta II, amino acids 2–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: Protein kinase Cb2 (PKCb2) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphosphate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 0.22mg/ml of enzyme in 20mM Tris/HCl pH7.5, 10mM benzamidine, 5% glycerol, 1mM EDTA, 1mM EGTA, 1mM PMSF, 0.1% 2-mercaptoethanol, 0.02% Triton X-100. Frozen solution.
• Bio-activity: 1170U/mg, where one unit of PKC beta II activity is defined as 1nmol phosphate incorporated into 0.1mg/ml histone H1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 78.2 kDa
• AA Sequence: 1 MHHHHHHEFA DPAAGPPPSE GEESTVRFAR KGALRQKNVH EVKNHKFTAR FFKQPTFCSH 61 CTDFIWGFGK QGFQCQVCCF VVHKRCHEFV TFSCPGADKG PASDDPRSKH KFKIHTYSSP 121 TFCDHCGSLL YGLIHQGMKC DTCMMNVHKR CVMNVPSLCG TDHTERRGRI YIQAHIDRDV 181 LIVLVRDAKN LVPMDPNGLS DPYVKLKLIP DPKSESKQKT KTIKCSLNPE WNETFRFQLK 241 ESDKDRRLSV EIWDWDLTSR NDFMGSLSFG ISELQKASVD GWFKLLSQEE GEYFNVPVPP 301 EGSEANEELR QKFERAKISQ GTKVPEEKTT NTVSKFDNNG NRDRMKLTDF NFLMVLGKGS 361 FGKVMLSERK GTDELYAVKI LKKDVVIQDD DVECTMVEKR VLALPGKPPF LTQLHSCFQT 421 MDRLYFVMEY VNGGDLMYHI QQVGRFKEPH AVFYAAEIAI GLFFLQSKGI IYRDLKLDNV 481 MLDSEGHIKI ADFGMCKENI WDGVTTKTFC GTPDYIAPEI IAYQPYGKSV DWWAFGVLLY 541 EMLAGQAPFE GEDEDELFQS IMEHNVAYPK SMSKEAVAIC KGLMTKHPGK RLGCGPEGER 601 DIKEHAFFRY IDWEKLERKE IQPPYKPKAC GRNAENFDRF FTRHPPVLTP PDQEVIRNID 661 QSEFEGFSFV NSEFLKPEVK S
• Purity: Purity 90.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKCB protein kinase C beta [ Homo sapiens (human) ]
• Official Symbol: PRKCB
• Synonyms: PRKCB; protein kinase C beta; PKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
• Gene ID: 5579
• mRNA Refseq: NM_002738.7
• Protein Refseq: NP_002729.2
• MIM: 176970
• UniProt ID: P05771