| Description : |
KPCZ (Protein kinase C, zeta type, nPKC-zeta, EC 2.7.1.37) is one of the major protein kinase C (PKC) isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphosphate, the former acting synergistically with Ca2+ to activate PKC, resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
2-end |
| Form : |
0.538mg/ml of enzyme in 20mM Tris/HCl pH7.5, 0.02% Triton X-100, 5% glycerol, 1mM EDTA, 1mM EGTA, 0.1% 2-mercaptoethanol, 1mM PMSF, 10mM benzamidine. Frozen solution. |
| Bio-activity : |
1377U/mg, where one unit of PKCζ activity is defined as 1nmol phosphate incorporated into 50μM PKCtide (ERMRPRKRQGSVRRRV) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
68.9 kDa |
| AA Sequence : |
1 MHHHHHHEFP SRTGPKMEGS GGRVRLKAHY GGDIFITSVD AATTFEELCE EVRDMCRLHQ 61 QHPLTLKWVD SEGDPCTVSS QMELEEAFRL ARQCRDEGLI IHVFPSTPEQ PGLPCPGEDK 121 SIYRRGARRW RKLYRANGHL FQAKRFNRRA YCGQCSERIW GLARQGYRCI NCKLLVHKRC 181 HGLVPLTCRK HMDSVMPSQE PPVDDKNEDA DLPSEETDGI AYISSSRKHD SIKDDSEDLK 241 PVIDGMDGIK ISQGLGLQDF DLIRVIGRGS YAKVLLVRLK KNDQIYAMKV VKKELVHDDE 301 DIDWVQTEKH VFEQASSNPF LVGLHSCFQT TSRLFLVIEY VNGGDLMFHM QRQRKLPEEH 361 ARFYAAEICI ALNFLHERGI IYRDLKLDNV LLDADGHIKL TDYGMCKEGL GPGDTTSTFC 421 GTPNYIAPEI LRGEEYGFSV DWWALGVLMF EMMAGRSPFD IITDNPDMNT EDYLFQVILE 481 KPIRIPRFLS VKASHVLKGF LNKDPKERLG CRPQTGFSDI KSHAFFRSID WDLLEKKQAL 541 PPFQPQITDD YGLDNFDTQF TSEPVQLTPD DEDAIKRIDQ SEFEGFEYIN PLLLSTEESV |
| Purity : |
Purity 91.8% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
