| Description : |
This protein is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and one intracytoplasmic catalytic domain, thus belongs to receptor type PTP. The extracellular region of this PTP is composed of multiple fibronectin type_III repeats, which was shown to interact with neuronal receptor and cell adhesion molecules, such as contactin and tenascin C. This protein was also found to interact with sodium channels, and thus may regulate sodium channels by altering tyrosine phosphorylation status. The functions of the interaction partners of this protein implicate the roles of this PTP in cell adhesion, neurite growth, and neuronal differentiation. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
GST |
| Protein length : |
1643-end |
| Form : |
0.538mg/ml of protein in 25mM Tris/HCl pH 8.0, 75mM NaCl, 10mM GSH, 50% glycerol, 0.5mM DTT. Frozen solution. |
| Bio-activity : |
12044U/mg, where one unit of PTPβ, active activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8- difluoro-4-methylumbelliferyl phosphate (DiFMUP) at room temperature. |
| Molecular Mass : |
69 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPGYP YDVPDYASPG 241 ASRQKVSHGR ERPSARLSIR RDRPLSVHLN LGQKGNRKTS CPIKINQFEG HFMKLQADSN 301 YLLSKEYEEL KDVGRNQSCD IALLPENRGK NRYNNILPYD ATRVKLSNVD DDPCSDYINA 361 SYIPGNNFRR EYIVTQGPLP GTKDDFWKMV WEQNVHNIVM VTQCVEKGRV KCDHYWPADQ 421 DSLYYGDLIL QMLSESVLPE WTIREFKICG EEQLDAHRLI RHFHYTVWPD HGVPETTQSL 481 IQFVRTVRDY INRSPGAGPT VVHCSAGVGR TGTFIALDRI LQQLDSKDSV DIYGAVHDLR 541 LHRVHMVQTE CQYVYLHQCV RDVLRARKLR SEQENPLFPI YENVNPEYHR DPVYSRH |
| Purity : |
Purity 87% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
