Active Recombinant Human ROS1(1883-end) Protein, N-His-tagged

• Cat.No.: ROS1-218H
• Product Overview: N-terminal 6Histagged recombinant human Ros residues 1883–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: Protein tyrosine kinase ROS is a role for morphogenic induction and proliferation of epithelial cells in the kidney, intestine and lung. ROS may provide a signal transduction pathway for epithelial-mesenchymal interactions.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1883-end
• Form: 3.638mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 271U/mg, where one unit of Ros, active activity is defined as 1nmol phosphate incorporated into 250μM (KKKSPGEYVNIEFG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 53.4 kDa
• AA Sequence: 1 MHHHHHHRHR RLKNQKSAKE GVTVLINEDK ELAELRGLAA GVGLANACYA IHTLPTQEEI 61 ENLPAFPREK LTLRLLLGSG AFGEVYEGTA VDILGVGSGE IKVAVKTLKK GSTDQEKIEF 121 LKEAHLMSKF NHPNILKQLG VCLLNEPQYI ILELMEGGDL LTYLRKARMA TFYGPLLTLV 181 DLVDLCVDIS KGCVYLERMH FIHRDLAARN CLVSVKDYTS PRIVKIGDFG LARDIYKNDY 241 YRKRGEGLLP VRWMAPESLM DGIFTTQSDV WSFGILIWEI LTLGHQPYPA HSNLDVLNYV 301 QTGGRLEPPR NCPDDLWNLM TQCWAQEPDQ RPTFHRIQNQ LQLFRNFFLN SIYQCRDEAN 361 NSGVINESFE GEDGDVICLN SDDIMPVALM ETKNREGLNY MVLATECGQG EEKSEGPLGS 421 QESESCGLRK EEKEPHADKD FCQEKQVAYC PSGKPEGLNY ACLTHSGYGD GSD
• Purity: Purity 81.6% by SDS-PAGE and Coomassie blue staining
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ROS1 ROS proto-oncogene 1, receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: ROS1
• Synonyms: ROS1; ROS proto-oncogene 1, receptor tyrosine kinase; ROS; MCF3; c-ros-1
• Gene ID: 6098
• mRNA Refseq: NM_002944.3
• Protein Refseq: NP_002935.2
• MIM: 165020
• UniProt ID: P08922