Active Recombinant Human SIK1(1-281) Protein, N-His-tagged

• Cat.No.: SIK1-227H
• Product Overview: N-terminal 6Histagged, recombinant, human SIK, amino acids 1–281, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: SIK plays a transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. This protein has a potential role in G2/M cell cycle regulation, and inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3 (By similarity).
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-281 aa
• Form: 0.918mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 451U/mg, where one unit of SIK, active activity is defined as 1nmol phosphate incorporated into 100μM (AMARAASAAALARRR) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 36.2 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MVIMSEFSAD PAGQGQGQQK PLRVGFYDIE 61 RTLGKGNFAV VKLARHRVTK TQVAIKIIDK TRLDSSNLEK IYREVQLMKL LNHPHIIKLY 121 QVMETKDMLY IVTEFAKNGE MFDYLTSNGH LSENEARKKF WQILSAVEYC HDHHIVHRDL 181 KTENLLLDGN MDIKLADFGF GNFYKSGEPL STWCGSPPYA APEVFEGKEY EGPQLDIWSL 241 GVVLYVLVCG SLPFDGPNLP TLRQRVLEGR FRIPFFMSQD CESLIRRMLV VDPARRITIA 301 QIRQHRWMRA E
• Purity: Purity 52% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SIK1 salt inducible kinase 1 [ Homo sapiens (human) ]
• Official Symbol: SIK1
• Synonyms: SIK1; salt inducible kinase 1; MSK; SIK; DEE30; SIK-1; SIK1B; SNF1LK
• Gene ID: 150094
• mRNA Refseq: NM_173354.5
• Protein Refseq: NP_775490.2
• MIM: 605705
• UniProt ID: P57059