Active Recombinant Human SNRK(1-505) Protein, C-His-tagged

• Cat.No.: SNRK-230H
• Product Overview: C-terminal 6His-tagged, recombinant, human SNRK amino acids 1-505 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: SNF-related serine/threonine-protein kinase may play a role in hematopoietic cell proliferation or differentiation. It is a potential mediator of neuronal apoptosis.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-505 aa
• Form: 2.231mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 210U/mg, where one unit of SNRK, active activity is defined as 1nmol phosphate incorporated into 250μM (KKLRRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 58 kDa
• AA Sequence: 1 MAGFKRGYDG KIAGLYDLDK TLGRGHFAVV KLARHVFTGE KVAVKVIDKT KLDTLATGHL 61 FQEVRCMKLV QHPNIVRLYE VIDTQTKLYL ILELGDGGDM FDYIMKHEEG LNEDLAKKYF 121 AQIVHAISYC HKLHVVHRDL KPENVVFFEK QGLVKLTDFG FSNKFQPGKK LTTSCGSLAY 181 SAPEILLGDE YDAPAVDIWS LGVILFMLVC GQPPFQEAND SETLTMIMDC KYTVPSHVSK 241 ECKDLITRML QRDPKRRASL EEIENHPWLQ GVDPSPATKY NIPLVSYKNL SEEEHNSIIQ 301 RMVLGDIADR DAIVEALETN RYNHITATYF LLAERILREK QEKEIQTRSA SPSNIKAQFR 361 QSWPTKIDVP QDLEDDLTAT PLSHATVPQS PARAADSVLN GHRSKGLCDS AKKDDLPELA 421 GPALSTVPPA SLKPTASGRK CLFRVEEDEE EDEEDKKPMS LSTQVVLRRK PSVTNRLTSR 481 KSAPVLNQIF EEGESDDEFD MDENLHHHHH H
• Purity: Purity 91% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SNRK SNF related kinase [ Homo sapiens (human) ]
• Official Symbol: SNRK
• Synonyms: SNRK; SNF related kinase; HSNFRK
• Gene ID: 54861
• mRNA Refseq: NM_017719.5
• Protein Refseq: NP_060189.3
• MIM: 612760
• UniProt ID: Q9NRH2