Active Recombinant Human SRPK1(2-end) Protein, N-GST-tagged

• Cat.No.: SRPK1-232H
• Product Overview: N-terminal GSTtagged, recombinant, human SRPK1, amino acids 2–end. Expressed in E. coli cells. Purified using glutathione agarose.

Specification

• Description: Two SR Protein-specific Kinases, SRPK1 and SRPK2, have been shown to phosphorylate specifically the RS motifs of the SR family of splicing factors. Phosphorylation of an SR protein (ASF/SF2) by either SRPk1 or 2 enhanced its interaction with another RS domain-containing protein, and overexpression of either kinase induced specific redistribution of splicing factors in the nucleus. SRPK1 is considered to be a constitutively active kinase predominantly active in testis. Inhibition of SRPK1 may render male germ cell and other tumors insensitive to cisplatin.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: 2-end
• Form: 0.417mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 4084U/mg, where one unit of SRPK1, active activity is defined as 1nmol phosphate incorporated into 300μM (RSRSRSRSRSRSRSR) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 101 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPLG SERKVLALQA 241 RKKRTKAKKD KAQRKSETQH RGSAPHSESD LPEQEEEILG SDDDEQEDPN DYCKGGYHLV 301 KIGDLFNGRY HVIRKLGWGH FSTVWLSWDI QGKKFVAMKV VKSAEHYTET ALDEIRLLKS 361 VRNSDPNDPN REMVVQLLDD FKISGVNGTH ICMVFEVLGH HLLKWIIKSN YQGLPLPCVK 421 KIIQQVLQGL DYLHTKCRII HTDIKPENIL LSVNEQYIRR LAAEATEWQR SGAPPPSGSA 481 VSTAPQPKPA DKMSKNKKKK LKKKQKRQAE LLEKRMQEIE EMEKESGPGQ KRPNKQEESE 541 SPVERPLKEN PPNKMTQEKL EESSTIGQDQ TLMERDTEGG AAEINCNGVI EVINYTQNSN 601 NETLRHKEDL HNANDCDVQN LNQESSFLSS QNGDSSTSQE TDSCTPITSE VSDTMVCQSS 661 STVGQSFSEQ HISQLQESIR AEIPCEDEQE QEHNGPLDNK GKSTAGNFLV NPLEPKNAEK 721 LKVKIADLGN ACWVHKHFTE DIQTRQYRSL EVLIGSGYNT PADIWSTACM AFELATGDYL 781 FEPHSGEEYT RDEDHIALII ELLGKVPRKL IVAGKYSKEF FTKKGDLKHI TKLKPWGLFE 841 VLVEKYEWSQ EEAAGFTDFL LPMLELIPEK RATAAECLRH PWLNS
• Purity: Purity 64.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SRPK1 SRSF protein kinase 1 [ Homo sapiens (human) ]
• Official Symbol: SRPK1
• Synonyms: SRPK1; SRSF protein kinase 1; SFRSK1
• Gene ID: 6732
• mRNA Refseq: NM_003137.5
• Protein Refseq: NP_003128.3
• MIM: 601939
• UniProt ID: Q96SB4