Active Recombinant Human SRPK2(46-end) Protein, N-GST-tagged

• Cat.No.: SRPK2-233H
• Product Overview: N-terminal GSTtagged, recombinant, human SRPK2, amino acids 46–end. Expressed in E. coli cells. Purified using glutathione agarose.

Specification

• Description: Two SR Protein-specific Kinases, SRPK1 and SRPK2, have been shown to phosphorylate specifically the RS motifs of the SR family of splicing factors. Phosphorylation of an SR protein (ASF/SF2) by either SRPK1 or 2 enhanced its interaction with another RS domain-containing protein, and overexpression of either kinase induced specific redistribution of splicing factors in the nucleus. SRPK2 contains a proline-rich sequence at its NH2 terminus, the capacity to interact with a WW domain protein in vitro. Inhibition of SRPK2 may render male germ cell and other tumors insensitive to cisplatin.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: 46-end
• Form: 0.182mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 5280U/mg, where one unit of SRPK2, active activity is defined as 1nmol phosphate incorporated into 300μM (RSRSRSRSRSRSRSR) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 100 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFP GRQEEEILGS 241 DDEEQEDPAD YCKGGYHPVK IGDLFNGRYH VIRKLGWGHF STVWLCWDMQ GKRFVAMKVV 301 KSAQHYTETA LDEIKLLKCV RESDPSDPNK DMVVQLIDDF KISGMNGIHV CMVFEVLGHH 361 LLKWIIKSNY QGLPVRCVKS IIRQVLQGLD YLHSKCKIIH TDIKPENILM CVDDAYVRRM 421 AAEATEWQKA GAPPPSGSAV STAPQQKPIG KISKNKKKKL KKKQKRQAEL LEKRLQEIEE 481 LEREAERKII EENITSAAPS NDQDGEYCPE VKLKTTGLEE AAEAETAKDN GEAEDQEEKE 541 DAEKENIEKD EDDVDQELAN IDPTWIESPK TNGHIENGPF SLEQQLDDED DDEEDCPNPE 601 EYNLDEPNAE SDYTYSSSYE QFNGELPNGR HKIPESQFPE FSTSLFSGSL EPVACGSVLS 661 EGSPLTEQEE SSPSHDRSRT VSASSTGDLP KAKTRAADLL VNPLDPRNAD KIRVKIADLG 721 NACWVHKHFT EDIQTRQYRS IEVLIGAGYS TPADIWSTAC MAFELATGDY LFEPHSGEDY 781 SRDEDHIAHI IELLGSIPRH FALSGKYSRE FFNRRGELRH ITKLKPWSLF DVLVEKYGWP 841 HEDAAQFTDF LIPMLEMVPE KRASAGECLR HPWLNS
• Purity: Purity 66.3% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SRPK2 SRSF protein kinase 2 [ Homo sapiens (human) ]
• Official Symbol: SRPK2
• Synonyms: SRPK2; SRSF protein kinase 2; SFRSK2
• Gene ID: 6733
• mRNA Refseq: NM_001278273.2
• Protein Refseq: NP_001265202.1
• MIM: 602980
• UniProt ID: P78362