Active Recombinant Human TAOK3(1-411) Protein, N-His-tagged

• Cat.No.: TAOK3-239H
• Product Overview: N-terminal 6Histagged, recombinant, human TAO3 amino acids 1–411. Expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: TAO3 is a serine/threonine protein kinase that activates the p38/MAPK14 stress-activated MAPK cascade but inhibits the basal activity of the MAPK8/JNK cascade. The encoded protein is a member of the GCK subfamily of STE20-like kinases. In response to DNA damage, TAO3 is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-411 aa
• Form: 2.788mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1084U/mg, where one unit of TAO3, active activity is defined as 1nmol phosphate incorporated into 0.8mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 50.2 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MRKGVLKDPE IADLFYKDDP EELFIGLHEI 61 GHGSFGAVYF ATNAHTSEVV AIKKMSYSGK QTHEKWQDIL KEVKFLRQLK HPNTIEYKGC 121 YLKEHTAWLV MEYCLGSASD LLEVHKKPLQ EVEIAAITHG ALHGLAYLHS HALIHRDIKA 181 GNILLTEPGQ VKLADFGSAS MASPANSFVG TPYWMAPEVI LAMDEGQYDG KVDIWSLGIT 241 CIELAERKPP LFNMNAMSAL YHIAQNDSPT LQSNEWTDSF RRFVDYCLQK IPQERPTSAE 301 LLRHDFVRRD RPLRVLIDLI QRTKDAVREL DNLQYRKMKK ILFQETRNGP LNESQEDEED 361 SEHGTSLNRE MDSLGSNHSI PSMSVSTGSQ SSSVNSMQEV MDESSSELVM MHDDESTINS 421 SSSVVHKKDH VFIRDEAGHG D
• Purity: Purity 87.4% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TAOK3 TAO kinase 3 [ Homo sapiens (human) ]
• Official Symbol: TAOK3
• Synonyms: TAOK3; TAO kinase 3; DPK; JIK; hKFC-A; MAP3K18
• Gene ID: 51347
• mRNA Refseq: NM_001346487.2
• Protein Refseq: NP_001333416.1
• MIM: 616711
• UniProt ID: Q9H2K8