Active Recombinant Human TLK2(388-end) Protein, N-His-tagged

• Cat.No.: TLK2-247H
• Product Overview: N-terminal 6Histagged, recombinant, 388–end, human TLK2, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: The Tousled-like kinases, first described in Arabidopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly. Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S- phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 388-end
• Form: 2.668mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 569U/mg, where one unit of TLK2, active activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 48.8 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQALGA HSLLRLTLAE YHEQEEIFKL 61 RLGHLKKEEA EIQAELERLE RVRNLHIREL KRIHNEDNSQ FKDHPTLNDR YLLLHLLGRG 121 GFSEVYKAFD LTEQRYVAVK IHQLNKNWRD EKKENYHKHA CREYRIHKEL DHPRIVKLYD 181 YFSLDTDSFC TVLEYCEGND LDFYLKQHKL MSEKEARSII MQIVNALKYL NEIKPPIIHY 241 DLKPGNILLV NGTACGEIKI TDFGLSKIMD DDSYNSVDGM ELTSQGAGTY WYLPPECFVV 301 GKEPPKISNK VDVWSVGVIF YQCLYGRKPF GHNQSQQDIL QENTILKATE VQFPPKPVVT 361 PEAKAFIRRC LAYRKEDRID VQQLACDPYL LPHIRKSVST SSPAGAAIAS TSGASNNSSS 421 N
• Purity: Purity 71.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TLK2 tousled like kinase 2 [ Homo sapiens (human) ]
• Official Symbol: TLK2
• Synonyms: TLK2; tousled like kinase 2; HsHPK; MRD57; PKU-ALPHA
• Gene ID: 11011
• mRNA Refseq: NM_006852.6
• Protein Refseq: NP_006843.2
• MIM: 608439
• UniProt ID: Q86UE8