| Description : |
TTBK2 (Tau tubulin kinase 2) is a serine/threonine protein kinase that is involved in a variety of cellular processes, including regulation of the cytoskeleton, intracellular transport, and neuronal development and function. TTBK2 has been shown to be involved in the regulation of microtubule dynamics and stability, and alterations in TTBK2 expression and activity have been linked to neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Research has suggested that TTBK2 may also play a role in cancer. TTBK2 has been shown to be overexpressed in several types of cancer, including breast cancer and glioblastoma, and high levels of TTBK2 expression have been associated with poor prognosis. Additionally, TTBK2 has been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
GST |
| Protein length : |
1-331 aa |
| Form : |
0.12 mg/ml of enzyme in 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
1170 U/mg, where one unit of TTBK2 activity is defined as 1 nmol phosphate incorporated into 2 mg/ml Casein per minute at 30 centigrade with a final ATP concentration of 100 μM. |
| Molecular Mass : |
65 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFK GLRRQMSGGG 241 EQPDILSVGI LVKERWKVLR KIGGGGFGEI YDALDMLTRE NVALKVESAQ QPKQVLKMEV 301 AVLKKLQGKD HVCRFIGCGR NDRFNYVVMQ LQGRNLADLR RSQSRGTFTI STTLRLGRQI 361 LESIESIHSV GFLHRDIKPS NFAMGRFPST CRKCYMLDFG LARQFTNSCG DVRPPRAVAG 421 FRGTVRYASI NAHRNREMGR HDDLWSLFYM LVEFVVGQLP WRKIKDKEQV GSIKERYDHR 481 LMLKHLPPEF SIFLDHISSL DYFTKPDYQL LTSVFDNSIK TFGVIESDPF DWEKTGNDGS 541 LTTTTTSTTP QLHTRLTPAA IGIANA |
| Purity : |
Purity 77% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
