Recombinant Mouse Tumor Necrosis Factor

Cat. No.: Tnf-371M
Product Overview: Recombinant Mouse Tnf is adisulfide-linked monomer protein, consisting of 157 amino acids and migratesas an approximately 17 kDa protein under reducing conditions, and waslyophilized from 0.2 μm filtered PBS solution, pH 7.0.
Description: Tnf is secreted bymacrophages, monocytes, neutrophils, T-cells, NK- cells following theirstimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF-alpha while CD8(+) cells secrete little or no TNF- alpha. Stimulatedperipheral neutrophilic granulocytes but also unstimulated cells and also anumber of transformed cell lines, astrocytes, microglial cells, smooth musclecells, and fibroblasts also secrete Tnf. Human milk also contains thisfactor. The synthesis of TNF-alpha is induced by many different stimuliincluding interferons, IL2, GM-CSF, SP, Bradykinin, Immune complexes,inhibitors of cyclooxygenase and PAF (platelet activating factor).
Source: Mouse
Host: E. coli
Purity: >97%, asdetermined by SDS-PAGE and HPLC.
Sequence: MSTESMIRDV ELAEEALPQK MGGFQNSRRC LCLSLFSFLL VAGATTLFCL LNFGVIGPQR DEKFPNGLPL ISSMAQTLTL RSSSQNSSDK PVAHVVANHQ VEEQLEWLSQ RANALLANGM DLKDNQLVVP ADGLYLVYSQ VLFKGQGCPD YVLLTHTVSR FAISYQEKVN LLSAVKSPCP KDTPEGAELK PWYEPIYLGG VFQLEKGDQL SAEVNLPKYL DFAESGQVYF GVIAL
Molecular Weight: Tnf is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately 17kDa.
Biological Activity: TheED(50) was determined cytolysis of murine L929 cells in the presence ofActinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 3 x107 units/mg.
Endotoxin: Endotoxin contentwas assayed using a LAL gel clot method. Endotoxin level was found to be lessthan 0.1 ng/µg(1EU/µg).
Reconstitution: Aquick spin of the vial followed by reconstitution in distilled water to aconcentration not less than 0.1 mg/mL. This solution can then be diluted intoother buffers.
Storage: Thelyophilized protein is stable for at least 2 years from date of receipt at-20°C. Upon reconstitution, this cytokine can be stored in working aliquots at2°C - 8°C for one month, or at -20°C for six months, with a carrier proteinwithout detectable loss of activity. Avoid repeated freeze/thaw cycles.
OfficialSymbol: Tnf
Pathways: Adipocytokinesignaling pathway; Adipogenesis; African trypanosomiasis; Allograft rejection;Alzheimer"s disease; Amoebiasis; Apoptosis; Asthma; Death Receptor Signalling;Diabetes pathways; Dilated cardiomyopathy; HTLV-I infection; Hepatitis C; Herpessimplex infection; Influenza A
Gene Name: Tnf tumor necrosis factor [ Mus musculus ]
Synonyms: Tnf; tumornecrosis factor; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; MGC151434;TNF-alpha; tumor ncrosis factor; TNF-a; TNF alpha; cachectin; tumor necrosisfactor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligandsuperfamily member 2
Gene ID: 21926
mRNA Refseq: NM_013693
Protein Refseq: NP_038721
UniProt ID: P06804
Function: cytokine activity; identicalprotein binding; protease binding; protein binding; transcription regulatoryregion DNA binding; tumor necrosis factor receptor binding

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