||Recombinant humanMSRA protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.
||MSRA (methioninesulfoxide reductase A ) belongs to the MsrA Met sulfoxide reductase family.This enzyme has an important function as a repair enzyme for proteins thathave been inactivated by oxidation. It catalyzes the reversibleoxidation-reduction of methionine sulfoxide in proteins to methionine. Inenzymology, a MSRA is an enzyme that catalyzes the chemical reaction. The 3substrates of this enzyme are peptide-L-methionine, thioredoxin disulfide,and H2O, whereas its two products are peptide-L-methionine (R)-S-oxide andthioredoxin
||Liquid. In 20 mMTris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 50mM NaCl
||26.2kDa (237aa),confirmed by MALDI-TOF
||> 90% by SDS -PAGE
||0.5mg/ml (determinedby Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMGNSAS NIVSPQEALP GRKEQTPVAA KHHVNGNRTV EPFPEGTQMA VFGMGCFWGAERKFWVLKGV YSTQVGFAGG YTSNPTYKEV CSEKTGHAEV VRVVYQPEHM SFEELLKVFW ENHDPTQGMRQGNDHGTQYR SAIYPTSAKQ MEAALSSKEN YQKVLSEHGF GPITTDIREG QTFYYAEDYH QQYLSKNPNGYCGLGGTGVS CPVGIKK
||Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.