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Recombinant Human Methionine Sulfoxide Reductase A, His-tagged

Cat. No.: MSRA-2407H
Product Overview: Recombinant humanMSRA protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.
Description: MSRA (methioninesulfoxide reductase A ) belongs to the MsrA Met sulfoxide reductase family.This enzyme has an important function as a repair enzyme for proteins thathave been inactivated by oxidation. It catalyzes the reversibleoxidation-reduction of methionine sulfoxide in proteins to methionine. Inenzymology, a MSRA is an enzyme that catalyzes the chemical reaction. The 3substrates of this enzyme are peptide-L-methionine, thioredoxin disulfide,and H2O, whereas its two products are peptide-L-methionine (R)-S-oxide andthioredoxin
Form: Liquid. In 20 mMTris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 50mM NaCl
Molecular Weight: 26.2kDa (237aa),confirmed by MALDI-TOF
Purity: > 90% by SDS -PAGE
Concentration: 0.5mg/ml (determinedby Bradford assay)
Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMGNSAS NIVSPQEALP GRKEQTPVAA KHHVNGNRTV EPFPEGTQMA VFGMGCFWGAERKFWVLKGV YSTQVGFAGG YTSNPTYKEV CSEKTGHAEV VRVVYQPEHM SFEELLKVFW ENHDPTQGMRQGNDHGTQYR SAIYPTSAKQ MEAALSSKEN YQKVLSEHGF GPITTDIREG QTFYYAEDYH QQYLSKNPNGYCGLGGTGVS CPVGIKK
Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.
Officialsymbol: MSRA
Gene Name: MSRA methionine sulfoxidereductase A [Homo sapiens]
Synonyms: methioninesulfoxide reductase A; EC 1.8.4.11; Peptide Met(O) reductase; cytosolicmethionine-S-sulfoxide reductase; Peptide-methionine (S)-S-oxide reductase;peptide met (O) reductase; Protein-methionine-S-oxide reductase; peptidemethionine sulfoxide reductase; PMSR; mitochondrial peptide methioninesulfoxide reductase; OTTHUMP00000115600; OTTHUMP00000224855;OTTHUMP00000224856; OTTHUMP00000224858; OTTHUMP00000229541;OTTHUMP00000230073
Gene ID: 4482
mRNA Refseq: NM_001135670
Protein Refseq: NP_001129142
MIM: 601250
UniProt ID: Q9UJ68
Chromosome Location: 8p23.1
Function: oxidoreductaseactivity; peptide-methionine-(S)-S-oxide reductase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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