||Recombinant mouse Pgam1protein,fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
||Pgam1 belongs to thephosphoglycerate mutase family. This protein is important components ofglucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes thereversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate(2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing,in different tissues, different proportions of a slow-migrating muscle (MM)isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB).
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT.
||31.4 kDa (278aa)confirmed by MALDI-TOF
||> 95% by SDS-PAGE
||1 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALRDAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWRRSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAHGNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
|Pathway||Gluconeogenesis; Glucose metabolism; Glycolysis;Glycolysis / Gluconeogenesis; Glycolysis and Gluconeogenesis; Glycolysis,core module involving three-carbon compounds; Metabolic pathways; gluconeogenesis;glycolysis I; glycolysis II; glycolysis III|