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Recombinant Mouse Phosphoglycerate Mutase 1, His-tagged

Cat. No.: Pgam1-1955M
Product Overview: Recombinant mouse Pgam1protein,fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
Description: Pgam1 belongs to thephosphoglycerate mutase family. This protein is important components ofglucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes thereversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate(2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing,in different tissues, different proportions of a slow-migrating muscle (MM)isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB).
Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT.
Molecular Weight: 31.4 kDa (278aa)confirmed by MALDI-TOF
Purity: > 95% by SDS-PAGE
Concentration: 1 mg/ml(determined by Bradford assay)
Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALRDAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWRRSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAHGNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK
Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
OfficialSymbol: Pgam1
nullValue_other:
PathwayGluconeogenesis; Glucose metabolism; Glycolysis;Glycolysis / Gluconeogenesis; Glycolysis and Gluconeogenesis; Glycolysis,core module involving three-carbon compounds; Metabolic pathways; gluconeogenesis;glycolysis I; glycolysis II; glycolysis III
Gene Name: Pgam1phosphoglycerate mutase 1 [ Mus musculus ]
Synonyms: Pgam1; phosphoglyceratemutase 1; Pgam-1;2310050F24Rik; PGAM-B; EC 3.1.3.13; EC 5.4.2.1; EC 5.4.2.4; BPG-dependent PGAM1; Phosphoglycerate mutase isozymeB
Gene ID: 18648
mRNA Refseq: NM_023418
Protein Refseq: NP_075907
UniProt ID: Q9DBJ1
Chromosome Location: 19

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