||Recombinant human NUDT9 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (47-350aa).
||NUDT9 belongs to a superfamily of Nudix hydrolases. It is known to function as a highlyspecific adenosine diphosphate ribose pyrophosphatase that hydrolyzes ADPriboseto AMP and ribose 5"-phosphate. It has been suggested thatNUDT9 may be involved in the regulation of the menstrual cycle and maybe related to the proliferation of glandular cells in the human endometrium.
||Liquid. In 20mM Tris-HClbuffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
||36.5 kDa (325aa), confirmed by MALDI-TOF(molecular weight on SDS-PAGE will appear higher)
||>95 % by SDS -PAGE
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MNTNVMSGSN GSKENSHNKA RTSPYPGSKV ERSQVPNEKV GWLVEWQDYK PVEYTAVSVL AGPRWADPQI SESNFSPKFN EKDGHVERKS KNGLYEIENG RPRNPAGRTG LVGRGLLGRW GPNHAADPII TRWKRDSSGN KIMHPVSGKH ILQFVAIKRK DCGEWAIPGG MVDPGEKISA TLKREFGEEA LNSLQKTSAE KREIEEKLHK LFSQDHLVIY KGYVDDPRNT DNAWMETEAV NYHDETGEIM DNLMLEAGDD AGKVKWVDIN DKLKLYASHS QFIKLVAEKR DAHWSEDSEA DCHAL
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.