Recombinant Human Enolase 3 (beta, muscle), His-tagged

• Cat.No.: ENO3-2498H
• Product Overview: Recombinant human ENO3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 49 kDa.

Specification

• Description: ENO3 is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. ENO3 play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl.
• Molecular Mass: 49 kDa (454aa), confirmed by MALDI-TOF
• Protein length: 1-434aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MAMQKIFARE IL DSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK GVLKAVENIN STLGPALLQK KLSVADQEKV DKFMIELDGT ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN PDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFKE AMRIGAEVYH HL KGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE FYRNGKYDLD FKSPDDPARH ITGEKLGELY KSFIKNYPVV SIEDPFDQDD WATWTSFLSG VNIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV TESIQACKLA QSNGWGVMVS HR SGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK AIFAGRKFRN PKAK
• Purity: >95% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ENO3 enolase 3 (beta, muscle) [ Homo sapiens ]
• Official Symbol: ENO3
• Synonyms: ENO3; enolase 3 (beta, muscle); enolase 3, (beta, muscle); beta-enolase; muscle-specific enolase; skeletal muscle enolase; 2-phospho-D-glycerate hydrolyase; MSE; GSD13
• Gene ID: 2027
• mRNA Refseq: NM_001193503
• Protein Refseq: NP_001180432
• MIM: 131370
• UniProt ID: P13929
• Chromosome Location: 17pter-p11
• Pathway: Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycolysis, organism-specific biosystem
• Function: lyase activity; magnesium ion binding; phosphopyruvate hydratase activity; protein heterodimerization activity; protein homodimerization activity