Recombinant Human Obg-Like ATPase 1, His-tagged

• Cat.No.: OLA1-2523H
• Product Overview: Recombinant human OLA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specification

• Description: OLA1 functions as a negative regulator of the cellular antioxidant response independent of transcriptional processes. OLA1 suppresses the antioxidant response through nontranscriptional mechanisms. Recombinant human OLA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl.
• Molecular Mass: 47.3 kDa (420aa), confirmed by MALDI-TOF
• Protein length: 1-396aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMPPKKG GDGIKPPPII GRFGTSLKIG IVGLPNVGKS TFFNVLTNSQ ASAENFPFCT IDPNESRVPV PDERFDFLCQ YHKPASKIPA FLNVVDIAGL VKGAHNGQGL GNAFLSHISA CDGIFHLTRA FEDDDITHVE GSVDPIRDIE IIHEELQLKD EEMIGPIIDK LEKVAVRGGD KKLKPEYDIM CKVKSWVIDQ KKPVRFYHDW NDKEIEVLNK HLFLTSKPMV YLVNLSEKDY IRKKNKWLIK IKEWVDKYDP GALVIPFSGA LELKLQELSA EERQKYLEAN MTQSALPKII KAGFAALQLE YFFTAGPDEV RAWTIRKGTK APQAAGKIHT DFEKGFIMAE VMKYEDFKEE GSENAVKAAG KYRQQGRNYI VEDGDIIFFK FNTPQQPKKK
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: OLA1 Obg-like ATPase 1 [ Homo sapiens ]
• Official Symbol: OLA1
• Synonyms: OLA1; Obg-like ATPase 1; GTP binding protein 9 (putative) , GTPBP9; obg-like ATPase 1; PTD004; GTP-binding protein PTD004; homologous yeast-44.2 protein; GTP-binding protein 9 (putative); DNA damage-regulated overexpressed in cancer 45 protein; DOC45; GBP45; GTBP9; GTPBP9; DKFZp313H1942
• Gene ID: 29789
• mRNA Refseq: NM_001011708
• Protein Refseq: NP_001011708
• MIM: 611175
• UniProt ID: Q9NTK5
• Chromosome Location: 2q31.1
• Function: ATP binding; GTP binding; hydrolase activity; nucleotide binding