Active Recombinant Mouse Bid protein(Met1-Asp195), His&GST-tagged
Cat.No. : | Bid-4029M |
Product Overview : | Recombinant Mouse BID (EDK99650.1) (Met 1-Asp 195) was expressed in E. coli, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
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Source : | E. coli |
Species : | Mouse |
Tag : | N-His&GST |
Protein length : | Met1-Asp195 |
Form : | Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.8. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.01 ng/mL. 3. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 7.1 ng/mL. |
Molecular Mass : | The recombinant mouse BID/GST chimera consisting of 435 amino acids and has a calculated molecular mass of 50 kDa. It migrates as a 47 kDa band in SDS-PAGE under reducing conditions. |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | Bid BH3 interacting domain death agonist [ Mus musculus ] |
Official Symbol : | Bid |
Synonyms : | BID; BH3 interacting domain death agonist; BH3-interacting domain death agonist; p22 BID; AI875481; AU022477; 2700049M22Rik; |
Gene ID : | 12122 |
mRNA Refseq : | NM_007544 |
Protein Refseq : | NP_031570 |
Products Types
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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