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Recombinant Human Glutamic-Pyruvate Transaminase (Alanine Aminotransferase), His-tagged

Cat. No.: GPT-1283H
Product Overview: Recombinant human GPT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Description: GPT, also known as alanine aminotransferases (ALT1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings.
Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 2mM DTT
Molecular Weight: 56.8 kDa (516aa), confirmed by MALDI-TOF
Purity: > 90% by SDS - PAGE
Concentration: 0.5 mg/ml (determined by Bradford assay)
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways: Alanine; Biosynthesis of alkaloids derived from ornithine; Carbon fixation in photosynthetic organisms; Metabolic pathways; Metabolism of amino acids
Gene Name: GPT glutamic-pyruvate transaminase (alanine aminotransferase) [ Homo sapiens ]
Official Symbol: GPT
Synonyms: GPT; glutamic-pyruvate transaminase (alanine aminotransferase); AAT1; ALT1; GPT1; Alanine aminotransferase 1; Glutamic--pyruvic transaminase 1; Glutamate pyruvate transaminase 1; EC 2.6.1.2; glutamic pyruvate transaminase
Gene ID: 2875
mRNA Refseq: NM_005309
Protein Refseq: NP_005300
MIM: 138200
UniProt ID: P24298
Chromosome Location: 8q24.3
Function: 1-aminocyclopropane-1-carboxylate synthase activity; L-alanine; 2-oxoglutarate aminotransferase activity; pyridoxal phosphate binding; transaminase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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