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Recombinant Human Hexokinase 1, His-tagged

Cat. No.: HK1-1141H
Product Overview: Recombinant human Hexokinase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Description: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency.
Concentration: 1 mg/ml
Source: Human
Host: E.coli
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, containing 10% glycerol.
Purity: > 90% by SDS - PAGE
Sequence: MGSSHHHHHH SSGLVPRGSH MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VG
Molecular Mass: 104.6 kDa (937aa)
Applications: SDS-PAGE
Bioactivity: Specific activity is 7-8 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH
Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Gene Name: HK1 hexokinase 1 [ Homo sapiens ]
Official Symbol: HK1
Synonyms: HK1; hexokinase 1; HK-I; HXK1; HK1-ta; HK1-tb; HK1-tc; Hexokinase-1; EC 2.7.1.1; Hexokinase type I; OTTHUMP00000019725; Brain form hexokinase; glycolytic enzyme
Gene ID: 3098
mRNA Refseq: NM_000188
Protein Refseq: NP_000179
MIM: 142600
UniProt ID: P19367
Chromosome Location: 10q22
Pathway: Amino sugar and nucleotide sugar metabolism; Biosynthesis of alkaloids derived from histidine and purine; Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic; Biosynthesis of alkaloids derived from shikimate pathway; Biosynthesis of alkaloids derived from terpenoid and polyketide; Biosynthesis of phenylpropanoids; Biosynthesis of plant hormones; Biosynthesis of terpenoids and steroids; Fructose and mannose metabolism; Galactose metabolism; Glycolysis / Gluconeogenesis; Insulin signaling pathway; Metabolic pathways; Starch and sucrose metabolism; Streptomycin biosynthesis; Type II diabetes mellitus; Metabolism of carbohydrates
Function: ATP binding; hexokinase activity; kinase activity; nucleotide binding; transferase activity

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