Recombinant Human HK1 Protein, GST-tagged

• Cat.No.: HK1-4821H
• Product Overview: Human HK1 full-length ORF ( AAH08730, 1 a.a. - 917 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 126.39 kDa
• AA Sequence: MIAAQLLAYYFTELKDDQVKKIDKY LYAMRLSDETLIDIMTRFRKEMKNG LSRDFNPTATVKMLPTFVRSIPDGS EKGDFIALDLGGSSFRILRVQVNHE KNQNVHMESEVYDTPENIVHGSGSQ LFDHVAECLGDFMEKRKIKDKKLPV GFTFSFPCQQSKIDEAILITWTKRF KASGVEGADVVKLLNKAIKKRGDYD ANIVAVVNDTVGTMMTCGYDDQHCE VGLIIGTGTNACYMEELRHIDLVEG DEGRMCINTEWGAFGDDGSLEDIRT EFDREIDRGSLNPGKQLFEKMVSGM YLGELVRLILVKMAKEGLLFEGRIT PELLTRGKFNTSDVSAIEKNKEGLH NAKEILTRLGVEPSDDDCVSVQHVC TIVSFRSANLVAATLGAILNRLRDN KGTPRLRTTVGVDGSLYKTHPQYSR RFHKTLRRLVPDSDVRFLLSESGSG KGAAMVTAVAYRLAEQHRQIEETLA HFHLTKDMLLEVKKRMRAEMELGLR KQTHNNAVVKMLPSFVRRTPDGTEN GDFLALDLGGTNFRVLLVKIRSGKK RTVEMHNKIYAIPIEIMQGTGEELF DHIVSCISDFLDYMGIKGPRMPLGF TFSFPCQQTSLDAGILITWTKGFKA TDCVGHDVVTLLRDAIKRREEFDLD VVAVVNDTVGTMMTCAYEEPTCEVG LIVGTGSNACYMEEMKNVEMVEGDQ GQMCINMEWGAFGDNGCLDDIRTHY DRLVDEYSLNAGKQRYEKMISGMYL GEIVRNILIDFTKKGFLFRGQISET LKTRGIFETKFLSQIESDRLALLQV RAILQQLGLNSTCDDSILVKTVCGV VSRRAAQLCGAGMAAVVDKIRENRG LDRLNVTVGVDGTLYKLHPHFSRIM HQTVKELSPKCNVSFLLSEDGSGKG AALITAVGVRLRTEASS
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -8 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: HK1 hexokinase 1 [ Homo sapiens ]
• Official Symbol: HK1
• Synonyms: HK1; hexokinase 1; hexokinase-1; HK I; glycolytic enzyme; hexokinase type I; brain form hexokinase; HKI; HXK1; HK1-ta; HK1-tb; HK1-tc
• Gene ID: 3098
• mRNA Refseq: NM_000188
• Protein Refseq: NP_000179
• MIM: 142600
• UniProt ID: P19367