Recombinant Human Leptin, His Tag

• Cat.No.: LEP-12H
• Product Overview: Recombinant human Leptin, expressed in E.Coli, with his tag.

Specification

• Description: Leptin is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is approximately~16 kDa in mass and encoded by the obese (ob)gene. leptin is expressed predominantly by adip centigradeytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cellsin the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymph centigradeytes and vascular endothelial cells.
• Source: E.Coli
• Species: Human
• Tag: His
• Form: The protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1mM DTT.
• Molecular Mass: Leptin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing amino acids 48-167 and having a total molecular mass of 19 kDa including the 4 kDa His tag. The Leptin is purified by proprietary chromatographic techniques.
• Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis bySDS-PAGE.
• Applications: 1. Positive control for Western blot. 2. Antibody production. 3. Protein assay.
• Usage: FOR RESEARCH USE ONLY
• Quality Control Test: Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0 centigrade. Reconstituted Leptin is best stored refrigerated at 4 centigrade.
• Reconstitution: The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.
• Warning: Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: LEP leptin [ Homo sapiens ]
• Official Symbol: LEP
• Synonyms: LEP; leptin; leptin (murine obesity homolog), leptin (obesity homolog, mouse), OB, OBS; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); OB; OBS; FLJ94114
• Gene ID: 3952
• mRNA Refseq: NM_000230
• Protein Refseq: NP_000221
• UniProt ID: P41159
• Chromosome Location: 7q31
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Developmental Biology, organism-specific biosystem; Diabetes pathways, organism-specific biosystem
• Function: growth factor activity; hormone activity; peptide hormone receptor binding