Recombinant Human RNASEH2A, His-tagged

• Cat.No.: RNASEH2A-7627H
• Product Overview: Recombinant human RNASEH2A protein, fused to His-tag at N-terminus, was expressed in E.coli

Specification

• Description: Ribonuclease H2 subunit A, also known as RNASEH2A, belongs to the RNase HII family and eukaryotic subfamily. Localized to the nucleus, RNASEH2A mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. RNASEH2A catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 35.8kDa (322aa)
• Protein length: 1-299aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDLSELE RDNTGRCRLS SPVPAVCRKE PCVLGVDEAG RGPVLGPMVY AICYCPLPRL ADLEALKVAD SKTLLESERE RLFAKMEDTD FVGWALDVLS PNLISTSMLG RVKYNLNSLS HDTATGLIQY ALDQGVNVTQ VFVDTVGMPE TYQARLQQSF PGIEVTVKAK ADALYPVVSA ASICAKVARD QAVKKWQFVE KLQDLDTDYG SGYPNDPKTK AWLKEHVEPV FGFPQFVRFS WRTAQTILEK EAEDVIWEDS ASENQEGLRK ITSYFLNEGS QARPRSSHRY FLERGLESAT SL
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: RNASEH2A ribonuclease H2, subunit A [ Homo sapiens ]
• Official Symbol: RNASEH2A
• Synonyms: RNASEH2A; ribonuclease H2, subunit A; Aicardi Goutieres syndrome 4 , ribonuclease H2, large subunit; ribonuclease H2 subunit A; AGS4; RNASEHI; RNHIA; RNHL; RNase H(35); RNase H2 subunit A; RNase HI large subunit; ribonuclease HI subunit A; ribonuclease HI large subunit; ribonuclease H2, large subunit; ribonuclease HI, large subunit; aicardi-Goutieres syndrome 4 protein; JUNB
• Gene ID: 10535
• mRNA Refseq: NM_006397
• Protein Refseq: NP_006388
• MIM: 606034
• UniProt ID: O75792
• Chromosome Location: 19p13.13
• Pathway: DNA replication, organism-specific biosystem; DNA replication, conserved biosystem
• Function: RNA binding; endonuclease activity; hydrolase activity; metal ion binding; ribonuclease H activity; ribonuclease activity