"RRP1" Related Products

Recombinant Human RRP1 293 Cell Lysate

Cat.No.: RRP1-2143HCL
Description: Antigen standard for ribosomal RNA processing 1 homolog (S. cerevisiae) (RRP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RRP1 ribosomal RNA processing 1 [ Homo sapiens ]
Official Symbol: RRP1
Synonyms: ribosomal RNA processing protein 1 homolog A; protein NNP-1; RRP1-like protein; novel nuclear protein 1; nucleolar protein Nop52; Nnp1 homolog, nucleolar protein; ribosomal RNA processing 1 homolog
Gene ID: 8568
mRNA Refseq: NM_003683
Protein Refseq: NP_003674
MIM: 610653
UniProt ID: P56182
Chromosome Location: 21q22.3
Function: poly(A) RNA binding

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