"RASSF1" Related Products

Recombinant Human RASSF1 293 Cell Lysate

Cat.No.: RASSF1-2499HCL
Description: Antigen standard for Ras association (RalGDS/AF-6) domain family member 1 (RASSF1), transcript variant A is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RASSF1 Ras association (RalGDS/AF-6) domain family member 1 [ Homo sapiens ]
Official Symbol: RASSF1
Synonyms: RASSF1; Ras association (RalGDS/AF-6) domain family member 1; ras association domain-containing protein 1; 123F2; NORE2A; RDA32; REH3P21; WUGSC:H_LUCA12.5; tumor suppressor protein RDA32; cardiac-specific ras association domain family 1 protein; pancreas-specific ras association domain family 1 protein; RASSF1A;
Gene ID: 11186
mRNA Refseq: NM_001206957
Protein Refseq: NP_001193886
MIM: 605082
UniProt ID: Q9NS23
Chromosome Location: 3p21.3
Pathway: Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Non-small cell lung cancer, organism-specific biosystem; Non-small cell lung cancer, conserved biosystem; Pathways in cancer, organism-specific biosystem; p53 pathway, organism-specific biosystem;
Function: identical protein binding; metal ion binding; protein N-terminus binding; protein binding; zinc ion binding;

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