"MASP2" Related Products

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Recombinant Human MASP2 293 Cell Lysate

Cat.No.: MASP2-4458HCL
Description: Antigen standard for mannan-binding lectin serine peptidase 2 (MASP2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MASP2 mannan-binding lectin serine peptidase 2 [ Homo sapiens ]
Official Symbol: MASP2
Synonyms: MASP2; mannan-binding lectin serine peptidase 2; mannan binding lectin serine peptidase 1 pseudogene 1 , mannan binding lectin serine protease 1 pseudogene 1 , mannan binding lectin serine protease 2 , MASP1P1; mannan-binding lectin serine protease 2; small MBL-associated protein; MBL-associated serine protease 2; MBL-associated plasma protein of 19 kD; mannan-binding lectin serine protease 1 pseudogene 1; mannose-binding protein-associated serine protease 2; mannan-binding lectin serine peptidase 1 pseudogene 1; sMAP; MAP19; MASP-2; MASP1P1;
Gene ID: 10747
mRNA Refseq: NM_006610
Protein Refseq: NP_006601
MIM: 605102
UniProt ID: O00187
Chromosome Location: 1p36.3-p36.2
Pathway: Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Creation of C4 and C2 activators, organism-specific biosystem; Immune System, organism-specific biosystem; Initial triggering of complement, organism-specific biosystem;
Function: calcium ion binding; calcium-dependent protein binding; peptidase activity; protein binding; serine-type endopeptidase activity;

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