"FLAD1" Related Products

Recombinant Human FLAD1 293 Cell Lysate

Cat.No.: FLAD1-6197HCL
Description: Antigen standard for FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) (FLAD1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: FLAD1 FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: FLAD1
Synonyms: FLAD1; FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae); Fad1, flavin adenine dinucleotide synthetase, homolog (yeast); FAD synthase; FAD1; PP591; FAD synthetase; FAD-synthetase; FAD pyrophosphorylase; FMN adenylyltransferase; flavin adenine dinucleotide synthase; flavin adenine dinucleotide synthetase; Fad1, flavin adenine dinucleotide synthetase, homolog; FADS; MGC31803; MGC40255; RP11-307C12.7;
Gene ID: 80308
mRNA Refseq: NM_001184891
Protein Refseq: NP_001171820
MIM: 610595
UniProt ID: Q8NFF5
Chromosome Location: 1q22
Pathway: Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of vitamins and cofactors, organism-specific biosystem; Metabolism of water-soluble vitamins and cofactors, organism-specific biosystem; Riboflavin metabolism, organism-specific biosystem; Riboflavin metabolism, conserved biosystem; Selenium Pathway, organism-specific biosystem;
Function: ATP binding; FMN adenylyltransferase activity; catalytic activity; nucleotide binding; nucleotidyltransferase activity; transferase activity;

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