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Recombinant Human WFDC2, His-tagged

Cat.No.: WFDC2-159H
Product Overview: Recombinant Human HE4 /WAP5 /WFDC2 Protein (rh HE4) Glu 31 - Phe 124 (Accession # AAH46106) was produced in human 293 cells (HEK293).
Description: Human Epididymis Protein 4 (HE4) is also known as WAP four-disulfide core domain protein 2 (WFDC2), Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, is a member of the WFDC domain family. HE4 / WFDC2 is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is also a small secretory protein, which may be involved in sperm maturation.
Source: HEK293
Species: Human
Tag: His
Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Molecular Mass: rh HE4 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 10.9 kDa. The predicted N-terminus is Glu 31. DTT-reduced Protein migrates as 19 kDa and 22-26 kDa in SDS-PAGE due to different glycosylation.
Endotoxin: Less than 1.0 EU per μg of the rh HE4 by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.
Gene Name: WFDC2 WAP four-disulfide core domain 2 [ Homo sapiens ]
Official Symbol: WFDC2
Synonyms: WFDC2; WAP four-disulfide core domain 2; WAP four-disulfide core domain protein 2; dJ461P17.6; EDDM4; epididymal protein 4; HE4; WAP5; epididymal secretory protein E4; putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; major epididymis-specific protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core; MGC57529;
Gene ID: 10406
mRNA Refseq: NM_006103
Protein Refseq: NP_006094
UniProt ID: Q14508
Chromosome Location: 20q12-q13.2
Function: endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity;

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