Recombinant Human Peptidylprolyl Isomerase H
Cat.No. : | PPIH-111H |
Product Overview : | Recombinant human cyclophilin H was expressed in E.coli and purified by conventional chromatography techniques, 19.2 kDa (177 aa). |
- Specification
- Gene Information
- Related Products
Cat. No. : | PPIH-111H |
Description : | Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-transisomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. The cyclophilin H is a specific component of the human U4/U6 small nuclear ribonucleoprotein particle involved in the nuclear splicing of premRNA.It stably associates with the U4/U6-60kD and -90kD proteins, the human orthologues of the Saccharomyces cerevisiaePrp4 and Prp3 splicing factors. |
Sequences of amino acids : | MAVANSSPVN PVVFFDVSIGG QEVGRMKIEL FADVVPKTAE NFRQFCTGEF RKDGVPIGYK GSTFHRVIKD FMIQGGDFVN GDGTGVASIY RGPFADENFK LRHSAPGLLS MANSGPSTN GCQFFITCSK CDWLDGKHV VFGKIIDGLL VMRKIENVPT GPNNKPKLPV V ISQCGEM |
Formulation : | Liquid in phosphate-buffered saline ( pH 7.4) containing 10% glycerol. |
Purity : | > 95% by SDS PAGE |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Biological activity : | Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. |
Activity Assay : | 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant Cyclophikin H (PPIH) protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C. |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Gene Name : | PPIH peptidylprolyl isomerase H (cyclophilin H) [ Homo sapiens ] |
Synonyms : | PPIH; CYPH; CYP-20; MGC5016; SnuCyp-20; USA-CYP; peptidylprolyl isomerase H (cyclophilin H); CYPH; CYP-20; MGC5016; USA-CYP; SnuCyp-20; peptidylprolyl isomerase H; PPIase h; rotamase H; cyclophilin H; USA-CyP SnuCyp-20; OTTHUMP00000008725; peptidylprolyl cis-trans isomerase H; U-snRNP-associated cyclophilin SunCyp-20; small nuclear ribonucleoprotein particlespecific cyclophilin H; CYP20; EC 5.2.1.8; Rotamase H; Small nuclear ribonucleoprotein particle-specific; peptidylprolyl isomerase H; peptidylprolyl isomerase H (cyclophilin H). |
Gene ID : | 10465 |
mRNA Refseq : | NM-006347 |
Protein Refseq : | NP-006338 |
MIM : | 606095 |
UniProt ID : | O43447 |
Chromosome Location : | 1p34.1 |
Function : | cyclosporin A binding, isomerase activity, isomeraseactivity |
Products Types
◆ Recombinant Protein | ||
Ppih-5043M | Recombinant Mouse Ppih Protein, Myc/DDK-tagged | +Inquiry |
PPIH-3367R | Recombinant Rhesus Macaque PPIH Protein, His (Fc)-Avi-tagged | +Inquiry |
PPIH-1980Z | Recombinant Zebrafish PPIH | +Inquiry |
PPIH-30685TH | Recombinant Human PPIH, T7 -tagged | +Inquiry |
PPIH-11421Z | Recombinant Zebrafish PPIH | +Inquiry |
◆ Lysates | ||
PPIH-2969HCL | Recombinant Human PPIH 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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