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Recombinant Human Peptidylprolyl Isomerase B (cyclophilin B)

Cat. No.: PPIB-63H
Product Overview: Recombinant human cyclophilin B was expressed in E.coli and purified by using conventional chromatography techniques, 21.2kDa (192aa).
Description: Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression.
Sequences of amino acids: MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE
Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 20 mM NaCl, 0.5 mM DTT, 10% glycerol
Purity: > 95% by SDS - PAGE
Concentration: 1 mg/ml (determined by Bradford assay)
Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method)
Biological activity: Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.
Activity Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant Cyclophilin B protein with 1ug in assay buffer.3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C.
Storage: Can be stored at +4°Cshort term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: PPIB peptidylprolyl isomerase B (cyclophilin B) [ Homo sapiens ]
Synonyms: SCYLP; CYPB; CYP-S1; MGC2224; MGC14109; PPIB; EC 5.2.1.8; PPIase; Rotamase; S-cyclophilin; rotamase; Cyclophilin B; cyclophilin-like protein; Peptidylprolyl isomerase B; peptidyl-prolyl cis-trans isomerase B; peptidylprolyl isomerase B (cyclophilin B); peptidylprolyl isomerase; cyclophilin [misleading, see comments]; peptide bond isomerase; peptidyl-prolyl cis-trans isomerase; peptidylproline cis-trans-isomerase
Gene ID: 5479
mRNA Refseq: NM_000942
Protein Refseq: NP_000933
MIM: 123841
UniProt ID: P23284
Chromosome Location: 15q21-q22
Function: isomerase activity; peptide binding; peptidyl-prolyl cis-trans isomerase activity; unfolded protein binding

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