Active Recombinant Human VEGFC, His tagged
Cat.No. : | VEGFC-211H |
Product Overview : | Recombinant Human VEGFC mature form (NP_005420.1) (Thr 103-Arg 227), fused with a C-terminal polyhistidine tag, was produced in Human Cell. |
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from sterile PBS, pH 7.4 |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at 2 μg/mL (100 μL/well) can bind VEGFR3 hFc, the EC50 of VEGFR3 hFc is 2-15 ng/mL. 2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM. 3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.1-0.5 μg/mL. 4. Captured VEGFR3/FLT4 Protein, Human on anti-Human IgG Fc via CM5 Chip can bind VEGF-C with an affinity constant of 31.82 pM as determined in a SPR assay (Biacore T200). |
Molecular Mass : | The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22.5 kDa due to glycosylation. |
Protein Length : | Thr103-Arg227 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | VEGFC vascular endothelial growth factor C [ Homo sapiens ] |
Official Symbol : | VEGFC |
Synonyms : | VEGFC; vascular endothelial growth factor C; VRP; VEGF-C; FLT4 ligand DHM; vascular endothelial growth factor-related protein; Flt4-L; |
Gene ID : | 7424 |
mRNA Refseq : | NM_005429 |
Protein Refseq : | NP_005420 |
MIM : | 601528 |
UniProt ID : | P49767 |
Products Types
◆ Recombinant Protein | ||
Vegfc-5535M | Recombinant Mouse Vascular Endothelial Growth Factor C | +Inquiry |
VEGFC-1319M | Acitve Recombinant Mouse/Rat VEGFC protein(Ala108-Arg223), Fc-tagged | +Inquiry |
VEGFC-160V | Active Recombinant Human VEGFC Protein (126 aa) | +Inquiry |
VEGFC-565H | Recombinant Human VEGFC Protein | +Inquiry |
VEGFC-4506H | Recombinant Human VEGFC Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
VEGFC-1306RCL | Recombinant Rat VEGFC cell lysate | +Inquiry |
VEGFC-2768HCL | Recombinant Human VEGFC cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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