Recombinant Human GCLM

• Cat.No.: GCLM-27451TH
• Product Overview: Recombinant full length Human GCLM with N terminal proprietary tag; Predicted MWt 56.25 kDa including the tag.

Specification

• Description: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
• Protein length: 274 amino acids
• Molecular Weight: 56.250kDa inclusive of tags
• Source: Wheat germ
• Tissue specificity: In all tissues examined. Highest levels in skeletal muscle.
• Form: Liquid
• Purity: Proprietary Purification
• Storage buffer: pH: 8.00Constituents:0.79% Tris HCl, 0.3% Glutathione
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHS EELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEK INPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVA QLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIV AIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDL TAFAKQFDIQLLTHNDPKELLSGASFQEALQESIPDIQAH EWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS
• Sequence Similarities: Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.

Gene Information

• Gene Name: GCLM glutamate-cysteine ligase, modifier subunit [ Homo sapiens ]
• Official Symbol: GCLM
• Synonyms: GCLM; glutamate-cysteine ligase, modifier subunit; GLCLR; glutamate--cysteine ligase regulatory subunit; gamma glutamylcysteine synthetase
• Gene ID: 2730
• mRNA Refseq: NM_002061
• Protein Refseq: NP_002052
• MIM: 601176
• Uniprot ID: P48507
• Chromosome Location: 1p21
• Pathway: Biological oxidations, organism-specific biosystem; Glutathione biosynthesis, glutamate => glutathione, organism-specific biosystem; Glutathione biosynthesis, glutamate => glutathione, conserved biosystem
• Function: contributes_to glutamate-cysteine ligase activity; contributes_to glutamate-cysteine ligase activity; glutamate-cysteine ligase catalytic subunit binding; protein heterodimerization activity