Recombinant Human GCLM Protein, GST-tagged

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Recombinant Human GCLM Protein, GST-tagged

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Cat.No. : GCLM-4802H
Product Overview : Human GCLM full-length ORF ( AAH41809, 1 a.a. - 274 a.a.) recombinant protein with GST-tag at N-terminal.
Description : Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 55.88 kDa
AA Sequence : MGTDSRAAKA LLARARTLHL QTGNLLNWGR LRKKCPSTHS EELHDCIQKT LNEWSSQINP DLVREFPDVL ECTVSHAVEK INPDEREEMK VSAKLFIVES NSSSSTRSAV DMACSVLGVA QLDSVIIASP PIEDGVNLSL EHLQPYWEEL ENLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL TAFAKQFDIQ LLTHNDPKEL LSGASFQEAL QESIPDIQAH EWVPLWLLRY SVIVKSRGII KSKGYILQAK RRGS
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : GCLM glutamate-cysteine ligase, modifier subunit [ Homo sapiens ]
Official Symbol : GCLM
Synonyms : GCLM; glutamate-cysteine ligase, modifier subunit; GLCLR; glutamate--cysteine ligase regulatory subunit; gamma glutamylcysteine synthetase; GCS light chain; GSC light chain; gamma-ECS regulatory subunit; glutamate--cysteine ligase modifier subunit; glutamate-cysteine ligase regulatory protein; gamma-glutamylcysteine synthetase regulatory subunit; glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD);
Gene ID : 2730
mRNA Refseq : NM_002061
Protein Refseq : NP_002052
MIM : 601176
UniProt ID : P48507

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