Recombinant Human GCLM Protein, GST-tagged

• Cat.No.: GCLM-4802H
• Product Overview: Human GCLM full-length ORF ( AAH41809, 1 a.a. - 274 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 55.88 kDa
• AA Sequence: MGTDSRAAKALLARARTLHLQTGNL LNWGRLRKKCPSTHSEELHDCIQKT LNEWSSQINPDLVREFPDVLECTVS HAVEKINPDEREEMKVSAKLFIVES NSSSSTRSAVDMACSVLGVAQLDSV IIASPPIEDGVNLSLEHLQPYWEEL ENLVQSKKIVAIGTSDLDKTQLEQL YQWAQVKPNSNQVNLASCCVMPPDL TAFAKQFDIQLLTHNDPKELLSGAS FQEALQESIPDIQAHEWVPLWLLRY SVIVKSRGIIKSKGYILQAKRRGS
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: GCLM glutamate-cysteine ligase, modifier subunit [ Homo sapiens ]
• Official Symbol: GCLM
• Synonyms: GCLM; glutamate-cysteine ligase, modifier subunit; GLCLR; glutamate--cysteine ligase regulatory subunit; gamma glutamylcysteine synthetase; GCS light chain; GSC light chain; gamma-ECS regulatory subunit; glutamate--cysteine ligase modifier subunit; glutamate-cysteine ligase regulatory protein; gamma-glutamylcysteine synthetase regulatory subunit; glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD)
• Gene ID: 2730
• mRNA Refseq: NM_002061
• Protein Refseq: NP_002052
• MIM: 601176
• UniProt ID: P48507