||Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL.
||The predicted molecular weight of Recombinant Human sFasR is 45 kDa.
|State Of Matter:
||>97% by SDS Page and analyzed by silver stain.
||<0.1 ng per 1 µg as determined by the LAL method.
||The biological activity of Human Soluble Fas Receptor is determined by its ability to inhibit the cytotoxicity of Jurkat cells. The expected ED50 for this effect is 10-15 mg/ml in the presence of 2 ng/ml of human FasL.
||This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.