Recombinant Human PPCDC, His-tagged

• Cat.No.: PPCDC-28672TH
• Product Overview: Recombinant full length Human PPCDC , with an N terminal His tag; 224 aa inclusive of tag, 24.6kDa.

Specification

• Description: Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC (EC 4.1.1.36), one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine (Daugherty et al.
• Protein length: 204 amino acids
• Conjugation: HIS
• Molecular Weight: 24.600kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, 1mM DTT, pH 8
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MEPKASCPAA APLMERKFHV LVGVTGSVAA LKLPLLVSKL LDIPGLEVAV VTTERAKHFY SPQDIPVTLY SDADEWEMWK SRSDPVLHID LRRWADLLLV APLDANTLGK VASGICDNLL TCVMRAWDRS KPLLFCPAMN TAMWEHPITA QQVDQLKAFG YVEIPCVAKK LVCGDEGLGA MAEVGTIVDK VKEVLFQHSG FQQS
• Sequence Similarities: Belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.

Gene Information

• Gene Name: PPCDC phosphopantothenoylcysteine decarboxylase [ Homo sapiens ]
• Official Symbol: PPCDC
• Synonyms: PPCDC; phosphopantothenoylcysteine decarboxylase; FLJ14585; MDS018
• Gene ID: 60490
• mRNA Refseq: NM_021823
• Protein Refseq: NP_068595
• MIM: 609854
• Uniprot ID: Q96CD2
• Chromosome Location: 15q24.2
• Pathway: Coenzyme A biosynthesis, organism-specific biosystem; Coenzyme A biosynthesis, pantothenate => CoA, organism-specific biosystem; Coenzyme A biosynthesis, pantothenate => CoA, conserved biosystem
• Function: lyase activity; phosphopantothenoylcysteine decarboxylase activity