||Recombinant human PSMG3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW = 15.2 kDa(142aa), confirmed by MALDI-TOF.
||PSMG3 is a chaperone protein which promotes assembly of the 20S proteasome. This protein may cooperate with PSMG1-PSMG2 heterodimers to orchestrate the correct assembly of proteasomes. It interacts with PSMG4, as well as directly alpha and beta subunits of the 20S proteasome but dissociates before the formation of half-proteasomes, probably upon recruitment of POMP.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MEDTPLVISK QKTEVVCGVP TQVVCTAFSS HILVVVTQFG KMGTLVSLEP SSVASDVSKP VLTTKVLLGQ DEPLIHVFAK NLVAFVSQEA GNRAVLLAVA VKDKSMEGLK ALREVIRVCQ VW
||> 90% by SDS – PAGE.
||Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
||0.5 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.