||Recombinant Human Nitric Oxide Synthase 1 (Neuronal) Adaptor Protein is producedinSf9 cells.MW=160kDa.
||This gene encodes a cytosolic protein that binds to the signaling molecule, neuronal nitric oxide synthase (nNOS). This protein has a C-terminal PDZ-binding domain that mediates interactions with nNOS and an N-terminal phosphotyrosine binding (PTB) domain that binds to the small monomeric G protein, Dexras1. Studies of the related mouse and rat proteins have shown that this protein functions as an adapter protein linking nNOS to specific targets, such as Dexras1 and the synapsins.
||Liquid. In 50mM triethanolamine, pH 7.5, 0.5mM EDTA, 7mM GSH, 0.2% CHAPSO, 1µm E64, 1µM leupeptin, 1µM pepstatin, 1mg/ml trypsin inhibitor and 10% glycerol.
||≥1nmol/100μl/min (U/100μl). Amount assayed 1µl. Optimal conditions: 37°C, pH 7.0, 1mM L-arginine, 1mM NADPH, 10µM (6R)-tetrahydrobiopterin, 5µM FAD, 5µM FMN, 50nM calmodulin, 1mM CaCl2 and 7mM GSH.
||-80°C. nNOS is relatively unstable. Keep stock vial on ice at all times when performing experiments! Enzyme loses approx. 10-15% of activity during a single freeze/thaw cycle.