Recombinant Full Length Human PRKACA Protein

• Cat.No.: PRKACA-149HFL
• Product Overview: Untagged, recombinant full-length human PKA, catalytic subunit type alpha, expressed in E.coli cells. Purified using phosphocellulose P11 followed by gel filtration.

Specification

• Description: Cyclic-AMP exerts its effects in animal cells mainly by activating the enzyme cyclic AMP-dependent protein kinase A (PKA). This enzyme catalyzes transfer of the terminal phosphate group from ATP to specific serines and threonines of selected proteins. Covalent phosphorylation of the appropriate amino acids of specific cellular proteins in turn results in altered cellular function as a consequence of activation or inactivation of enzymes or altered membrane permeability to ions or other possible effects. PKA is found in all animal cells, and it is known that hormones or transmitters may be mediated by cAMP-stimulated phosphorylation of specific sites on neuronal or non-neuronal cells to exert their physiological function.
• Source: E. coli
• Species: Human
• Protein length: Full Length
• Form: 0.432mg/ml of enzyme in 30mM potassium phosphate pH7.4, 150mM KCl, 1mM EDTA, 1mM DTT, 50% glycerol. Frozen solution.
• Bio-activity: 8580U/mg, where one unit of PKA activity is defined as 1nmol phosphate incorporated into 30μM Kemptide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 41 kDa
• AA Sequence: 1 MGNAAAAKKG SEQESVKEFL AKAKEDFLKK WESPAQNTAH LDQFERIKTL GTGSFGRVML 61 VKHKETGNHY AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVKLEFS FKDNSNLYMV 121 MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDQQGY 181 IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF 241 ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVN DIKNHKWFAT 301 TDWIAIYQRK VEAPFIPKFK GPGDTSNFDD YEEEEIRVSI NEKCGKEFSE F
• Purity: Purity 97.8% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKACA protein kinase cAMP-activated catalytic subunit alpha [ Homo sapiens (human) ]
• Official Symbol: PRKACA
• Synonyms: PRKACA; protein kinase cAMP-activated catalytic subunit alpha; CAFD1; PKACA; PPNAD4
• Gene ID: 5566
• mRNA Refseq: NM_207518.3
• Protein Refseq: NP_997401.1
• MIM: 601639
• UniProt ID: P17612