Recombinant Human ITGA8 & ITGB1 protein(Met1-Leu1012 & Met1-Asp728), Flag&His-tagged

• Cat.No.: ITGA8 & ITGB1-1171H
• Product Overview: Recombinant Human ITGA8 (P53708)(Met 1-Leu 1012) was fused with a flag tag at the C-terminus, constructed the plasmid 1 ; Recombinant Human ITGB1 (P05556-1)(Met 1-Asp 728) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-Flag&His
• Protein length: Met1-Leu1012 & Met1-Asp728
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant heterodimer of human ITGA8/ITGB1 comprises 1765 (1016 + 749) amino acids and has a calculated molecular mass of 196 (113 + 83) kDa. As a result of glycosylation, the apparent molecular mass of rh ITGA8/ITGB1 heterodimer is approximately 120 &140 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA8 integrin, alpha 8 [ Homo sapiens ]
• Official Symbol: ITGA8
• Synonyms: ITGA8; integrin, alpha 8; integrin alpha-8
• Gene ID: 8516
• mRNA Refseq: NM_003638
• Protein Refseq: NP_003629
• MIM: 604063
• UniProt ID: P53708