| Description : |
Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
146.7 kDa |
| AA Sequence : |
MKRNGSRNCLNRRSRFGSRERDWLR EDVKRGCVYLYGADTTTATTTTTTS SSSSSSSSSSDLHLVLCTVETPASE ICAGEGRESLYLQLHGDLVRRLEPT ERPLQIVYDYLSRLGFDDPVRIQEE ATNPDLGCMIRFYGEKPCHMDRLDR ILLSGIYNVRKGKTQLHKWAERLVV LCGTCLIVSSVKDCQTGKMHILPLV GGKIEEVKRRQYSLAFSSAGAQAQT YHVSFETLAEYQRWQRQASKVVSQR ISTVDLSCYSLEEVPEHLFYSQDIT YLNLRHNFMQLERPGGLDTLYKFSQ LKGLNLSHNKLGLFPILLCEISTLT ELNLSCNGFHDLPSQIGNLLNLQTL CLDGNFLTTLPEELGNLQQLSSLGI SFNNFSQIPEVYEKLTMLDRVVMAG NCLEVLNLGVLNRMNHIKHVDLRMN HLKTMVIENLEGNKHITHVDLRDNR LTDLDLSSLCSLEQLHCGRNQLREL TLSGFSLRTLYASSNRLTAVNVYPV PSLLTFLDLSRNLLECVPDWACEAK KIEVLDVSYNLLTEVPVRILSSLSL RKLMLGHNHVQNLPTLVEHIPLEVL DLQHNALTRLPDTLFSKALNLRYLN ASANSLESLPSACTGEESLSMLQLL YLTNNLLTDQCIPVLVGHLHLRILH LANNQLQTFPASKLNKLEQLEELNL SGNKLKTIPTTIANCKRLHTLVAHS NNISIFPEILQLPQIQFVDLSCNDL TEILIPEALPATLQDLDLTGNTNLV LEHKTLDIFSHITTLKIDQKPLPTT DSTVTSTFWSHGLAEMAGQRNKLCV SALAMDSFAEGVGAVYGMFDGDRNE ELPRLLQCTMADVLLEEVQQSTNDT VFMANTFLVSHRKLGMAGQKLGSSA LLCYIRPDTADPASSFSLTVANVGT CQAVLCRGGKPVPLSKVFSLEQDPE EAQRVKDQKAIITEDNKVNGVTCCT RMLGCTYLYPWILPKPHISSTPLTI QDELLILGNKALWEHLSYTEAVNAV RHVQDPLAAAKKLCTLAQSYGCQDN VGAMVVYLNIGEEGCTCEMNGLTLP GPVGFASTTTIKDAPKPATPSSSSG IASEFSSEMSTSEVSSEVGSTASDE HNAGGLDTALLPRPERRCSLHPTPT SGLFQRQPSSATFSSNQSDNGLDSD DDQPVEGVITNGSKVEVEVDIHCCR GRDLENSPPLIESSPTLCSEEHARG SCFGIRRQNSVNSGMLLPMSKDRME LQKSPSTSCLYGKKLSNGSIVPLED SLNLIEVATEVPKRKTGYFAAPTQM EPEDQFVVPHDLEEEVKEQMKQHQD SRLEPEPHEEDQTEPPEEFDTALTR TRPLEQKLISEEDLAANDILDYKDD DDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
