Recombinant Human PRKAA1 complex Protein, N-Flag-tagged

• Cat.No.: PRKAA1-06H
• Product Overview: Recombinant PRKAA1 Complex that includes full length human PRKAA1 protein (accession number NP_996790.3) with a N-FLAG tag and full length human PRKAB2 protein (accession number NP_005390.1) without tag and full length human PRKAG1 protein (accession number NP_002724.1) without tag was expressed in Sf9 cells. The molecular weights of PRKAA1, PRKAB2, PRKAG1 are 65.3 kDa, 30 kDa and 37.6 kDa, respectively

Specification

• Description: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Source: Baculovirus
• Species: Human
• Tag: N-Flag
• Molecular Mass: PRKAA1: 65.3 kDa
  PRKAB2: 30 kDa
  PRKAG1: 37.6 kDa
• Purity: > 90%
• Notes: This product is for research use only and is not for use in diagnostic procedures.
• Stability: This product is guaranteed for 6 months from date of arrival.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.6 μg/μL
• Storage Buffer: Recombinant PRKAA1 Complex is supplied 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5 mM TCEP.

Gene Information

• Gene Name: PRKAA1 protein kinase AMP-activated catalytic subunit alpha 1 [ Homo sapiens (human) ]
• Official Symbol: PRKAA1
• Synonyms: PRKAA1; protein kinase AMP-activated catalytic subunit alpha 1; AMPK; AMPKa1; AMPK alpha 1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; ACACA kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK subunit alpha-1; AMPK, alpha, 1; PRKAB2; protein kinase AMP-activated non-catalytic subunit beta 2; 5'-AMP-activated protein kinase subunit beta-2; 5'-AMP-activated protein kinase, beta-2 subunit; AMP-activated protein kinase beta 2 non-catalytic subunit; AMPK beta 2; AMPK beta-2 chain; AMPK subunit beta-2; protein kinase, AMP-activated, beta 2 non-catalytic subunit; PRKAG1; protein kinase AMP-activated non-catalytic subunit gamma 1; AMPKG; 5'-AMP-activated protein kinase subunit gamma-1; 5'-AMP-activated protein kinase, gamma-1 subunit; AMPK gamma-1 chain; AMPK gamma1; protein kinase, AMP-activated, gamma 1 non-catalytic subunit
• Gene ID: 5562
• mRNA Refseq: NM_206907
• Protein Refseq: NP_996790
• MIM: 602739
• UniProt ID: Q13131