Active Recombinant Human ROBO1 Protein, Fc-tagged

• Cat.No.: ROBO1-001H
• Product Overview: Recombinant Human ROBO1/DUTT1 (Ser20-Pro858) protein with Human IgG1 (Pro100-Lys330) at C-terminus was expressed in Mouse myeloma cell line.

Specification

• Description: Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Bio-activity: Measured by its binding ability in a functional ELISA. When recombinant human Slit3 is coated at 250 ng/mL, Recombinant Human ROBO1 Fc Chimera binds with a typical ED50 of 100-600 ng/mL.
• Molecular Mass: 119 kDa
• N-terminal Sequence Analysis: Ser20
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: ROBO1 roundabout guidance receptor 1 [ Homo sapiens (human) ]
• Official Symbol: ROBO1
• Synonyms: ROBO1; roundabout guidance receptor 1; SAX3; DUTT1; roundabout homolog 1; deleted in U twenty twenty; roundabout, axon guidance receptor, homolog 1
• Gene ID: 6091
• mRNA Refseq: NM_133631
• Protein Refseq: NP_598334
• MIM: 602430
• UniProt ID: Q9Y6N7