Recombinant Mouse CD1D2 & B2M protein(Met1-Gly305 & Met1-Met119), His-tagged

• Cat.No.: CD1D2 & B2M-3389M
• Product Overview: Recombinant Mouse CD1D2 (Met1-Gly305) was expressed in HEK293 with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Mouse B2M (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the Mouse CD1D2/B2M heterodimer was purified.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Gly305 & Met1-Met119
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant heterodimer of mouse CD1D2&B2M comprises 394 (295+99) amino acids and has a calculated molecular mass of 45.4 (33.7+11.7) KDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.